829 Long non-coding RNA HSFY2-10:1 functions as a ceRNA to promote keratinocyte proliferation and psoriatic inflammation through the miR-145-5p/FN1 axis

Psoriasis is a chronic autoimmune inflammatory disease caused by keratinocyte hyperproliferation and cell infiltration due to dysregulated immune system. Recently, the importance of epigenetic regulation has been increasingly recognized in psoriasis. However, few current studies have reported on mutual between lncRNA miRNA The main objective this study was explore role HSFY2-10:1/miR-145-5p/FN1 axis Using microarray, we identified that HSFY2-10:1 highly expressed psoriasis tissues compared with normal skin, further confirmed quantitative real-time PCR. By using an approach knockdown overexpression HSFY2-10:1, showed could promote proliferation increase secretion chemokine CCL20 M5 (IL-17A, IL-22, IL-1α, oncostatin M, TNF-α) induced human epidermal keratinocytes (NHEKs) HaCaT cells. Mechanistically, found act as CeRNA sponging miR-145-5p regulate FN1 expression keratinocytes. elevated lnc-HSFY2-10:1 overexpression, which obviously reversed presence mimics. Similarly, promotion viability significantly upon overexpression. We concluded levels via competitive binding miR-145-5p， may serve potential therapeutic candidate for treatment.